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微波_超聲協同輔助提取靈芝多糖工藝

52
2010, Vol. 31, No. 16
食品科學
※工藝技術
微波 - 超聲協同輔助提取靈芝多糖工藝
黃生權 1 , 2 ,李進偉 3 ,寧正祥 1 , *
(1.華南理工大學輕工與食品學院,廣東 廣州 510640;2. 無限極(中國)有限公司,廣東 江門 214122) 529156; 3. 江南大學食品學院,江蘇 無錫
摘 要:分析不品種和來源的 5 種靈芝的主要成分,確定浙江赤靈芝作為提取靈芝多糖的較佳原料。採用響應面 優化超聲 - 微波協同萃取法提取靈芝多糖的最佳工藝條件,分析超聲 - 微波協同萃取法對靈芝纖維結構的影響,比 較傳統水浴浸提法和超聲 - 微波協同萃取法對靈芝多糖提取率和結構的影響。結果表明:超聲 - 微波協同萃取最佳 的提取工藝條件為原料用量 100g,微波功率 284W,提取時間 12min,料液比(g/mL)1:11.6;與傳統水浴浸提法相 比,超聲 - 微波協同萃取法在較短的超聲提取時間下,靈芝多糖的提取率從 1.517% 提高到了 3.27%。 關鍵詞:超聲 - 微波協同萃取;傳統水浴浸提法;靈芝多糖;提取率
Ultrasonic-microwave Synergistic Extraction of Polysaccharides from Cultivated Ganoderma lucidum
HUANG Sheng-quan 1,2,LI Jin-wei3,NING Zheng-xiang 1, * (1. College of Light Industry and Food Science, South China University of Technology, Guangzhou Co. Ltd., Jiangmen 510640, China ; 2. Infinitus 214122, China) 529156, China ;3. School of Food Science and Technology, Jiangnan University, Wuxi
Abstract :Different cultivated species of Ganoderma from different geographic origins were analyzed for their chemical components, and Ganoderma lucidum from Zhejiang province was found to contain higher content of non-reducing sugars and lower contents of protein and ash and therefore, it was the selected raw material for extracting polysaccharides. The extraction process with water as extraction solvent on an ultrasonic-microwave synergistic extractor was optimized using response surface methodology based on Box-Behnken experimental design. Meanwhile, the structural change of Ganoderma lucidum before and after extraction was observed under scan electron microscope (SEM) and the extraction method developed in this study and the conventional water bath extraction method were compared for differences in the extraction yield of polysaccharides and the infrared spectral characteristics of the extraction products. The optimum conditions for the ultrasonic-microwave synergistic extraction of polysaccharides were found to be: raw material amount 100 g, microwave power 284 W, extraction duration 12 min, and material-to-liquid ratio 1:11.6 (g/mL). Ultrasonic-microwave synergistic extraction was time saving and gave a higher extraction yield of polysaccharides, 3.27%, when compared with the conventional water bath extraction method (115.6% extraction yield of polysaccharides). Key words:ultrasonic-microwave synergistic extraction;conventional water bath extraction;Ganoderma lucidum polysaccharides;yield 中圖分類號:Q539 文獻標識碼:A 文章編號:1002-6630(2010)16-0052-04
靈芝(Ganoderma lucidum) 為擔子菌門擔子菌綱多 孔菌科靈芝屬的一種藥用真菌,又稱靈芝草、仙草、 瑞 草 ,也 叫 長 生 草 、 靈 草 、還 魂 草 ,在 我 國 有 悠 久 的藥用歷史。我國古人認為靈芝是一種超自然的神藥仙 草,東漢時期的《神農本草經》對靈芝的功效有詳細 的極為肯定的記載,認為靈芝具有扶正固本、滋補強 壯、延年益壽等功效。現代藥理學與臨牀實踐進一步 證實了靈芝能抗衰老、增強肌體免疫力,而且具有廣
收稿日期:2009-11-01
譜的抗病毒、抗腫瘤作用。現代科學研究表明靈芝藥 理活性與其生物活性成分密切相關。近年來,對靈芝 營養成分的研究逐漸受到人們的重視,研究結果表明, 靈芝的營養成分種類繁多,除含有六大營養成分還含有 生理活性極高的多糖 [ 1 -7 ] 。 水浴浸提法是食品功能因素常用的提取方法[ 8 - 1 1 ] , 但水浴提取法存在提取時間長、提取率低、提取温度 高等缺點。近年來,超聲 - 微波協同萃取技術作為一種
作者簡介:黃生權(1978 —),男,高級工程師,博士,研究方向為天然產物提取及功能食品。E-mail:huangfeihong0220@//xw.yuguniang.net/doc/3f98bc97dd88d0d233d46a72.html * 通信作者:寧正祥(1962 —),男,教授,博士,研究方向為天然產物提取及功能食品。E-mail:fezhning@//xw.yuguniang.net/doc/3f98bc97dd88d0d233d46a72.html